[HTML][HTML] Three-dimensional type I collagen lattices induce coordinate expression of matrix metalloproteinases MT1-MMP and MMP-2 in microvascular endothelial cells
TL Haas, SJ Davis, JA Madri - Journal of Biological Chemistry, 1998 - ASBMB
Matrix metalloproteinases (MMPs) are hypothesized to play a key role in the processes of
endothelial cell migration and matrix remodeling during angiogenesis. We utilized an in vitro
model of microvascular endothelial cell angiogenesis, cells cultured within a collagen
matrix, to investigate the MMP profile of endothelial cells undergoing angiogenesis. We
demonstrated by gelatin zymography that monolayer cultures (two-dimensional) of
endothelial cells constitutively expressed low levels of latent MMP-2, but that culture in a …
endothelial cell migration and matrix remodeling during angiogenesis. We utilized an in vitro
model of microvascular endothelial cell angiogenesis, cells cultured within a collagen
matrix, to investigate the MMP profile of endothelial cells undergoing angiogenesis. We
demonstrated by gelatin zymography that monolayer cultures (two-dimensional) of
endothelial cells constitutively expressed low levels of latent MMP-2, but that culture in a …