[HTML][HTML] Anti-immunoglobulin-induced apoptosis in WEHI 231 cells involves the slow formation of ceramide from sphingomyelin and is blocked by bcl-XL
DA Wiesner, JP Kilkus, AR Gottschalk… - Journal of Biological …, 1997 - ASBMB
Prolonged (> 24 h) exposure to anti-IgM (an antigen surrogate that induces membrane cross-
linking and apoptosis) induced a 3-fold increase in the mass of endogenous ceramide
measured by 32 P labeling by diacylglycerol kinase and a 4-fold increase in ceramide as
measured by metabolic labeling with [3 H] palmitate in a B-lymphocyte cell line, WEHI 231.
This correlated with the induction of apoptosis. Shorter exposure times to anti-IgM (up to 8 h)
failed to elicit apoptosis and did not elicit increased ceramide formation. After 8 h, apoptosis …
linking and apoptosis) induced a 3-fold increase in the mass of endogenous ceramide
measured by 32 P labeling by diacylglycerol kinase and a 4-fold increase in ceramide as
measured by metabolic labeling with [3 H] palmitate in a B-lymphocyte cell line, WEHI 231.
This correlated with the induction of apoptosis. Shorter exposure times to anti-IgM (up to 8 h)
failed to elicit apoptosis and did not elicit increased ceramide formation. After 8 h, apoptosis …