[HTML][HTML] CD95/Fas-induced ceramide formation proceeds with slow kinetics and is not blocked by caspase-3/CPP32 inhibition
AD Tepper, JGR Boesen-de Cock, E de Vries… - Journal of Biological …, 1997 - ASBMB
The current confusion regarding the relevance of endogenous ceramide in mediating
CD95/Fas-induced apoptosis is based mainly on (i) discrepancies in kinetics of the
ceramide response between different studies using the same apoptotic stimulus and (ii) the
observation that late ceramide formation (hours) often parallels apoptosis onset. We
investigated CD95-induced ceramide formation in Jurkat cells, using two methods
(radiolabeling/thin layer chromatography and benzoylation/high performance liquid …
CD95/Fas-induced apoptosis is based mainly on (i) discrepancies in kinetics of the
ceramide response between different studies using the same apoptotic stimulus and (ii) the
observation that late ceramide formation (hours) often parallels apoptosis onset. We
investigated CD95-induced ceramide formation in Jurkat cells, using two methods
(radiolabeling/thin layer chromatography and benzoylation/high performance liquid …