[32] Characteristics of rabbit anti-PGH synthase antibodies and use in immunocytochemistry

WL Smith, TE Rollins - Methods in enzymology, 1982 - Elsevier
Publisher Summary This chapter discusses the characteristics of rabbit anti-PGH synthase
antibodies and its use in immunocytochemistry. Rabbit anti-PGH synthase sera when tested
by standard Ouchterlony double-diffusion analyses show single lines of precipitation with
samples of both solubilized sheep vesicular gland microsomes and pure PGH synthase; a
zone of identity is present between the lines formed with microsomal and purified PGH
synthases. When tested by immunoelectrophoresis, the purified cyclooxygenase shows an …