[HTML][HTML] Dissecting the interaction between nitric oxide synthase (NOS) and caveolin: Functional significance of the NOS caveolin binding domainin vivo
G Garcı́a-Cardeña, P Martasek, BSS Masters… - Journal of Biological …, 1997 - ASBMB
Endothelial nitric oxide synthase (eNOS) is a dually acylated peripheral membrane protein
that targets to the Golgi region and caveolae of endothelial cells. Recent evidence has
shown that eNOS can co-precipitate with caveolin-1, the resident coat protein of caveolae,
suggesting a direct interaction between these two proteins. To test this idea, we examined
the interactions of eNOS with caveolin-1 in vitro and in vivo. Incubation of endothelial cell
lysates or purified eNOS with glutathione S-transferase (GST)-caveolin-1 resulted in the …
that targets to the Golgi region and caveolae of endothelial cells. Recent evidence has
shown that eNOS can co-precipitate with caveolin-1, the resident coat protein of caveolae,
suggesting a direct interaction between these two proteins. To test this idea, we examined
the interactions of eNOS with caveolin-1 in vitro and in vivo. Incubation of endothelial cell
lysates or purified eNOS with glutathione S-transferase (GST)-caveolin-1 resulted in the …