[HTML][HTML] Direct interaction of endothelial nitric-oxide synthase and caveolin-1 inhibits synthase activity
H Ju, R Zou, VJ Venema, RC Venema - Journal of Biological Chemistry, 1997 - ASBMB
Endothelial nitric-oxide synthase (eNOS) and caveolin-1 are associated within endothelial
plasmalemmal caveolae. It is not known, however, whether eNOS and caveolin-1 interact
directly or indirectly or whether the interaction affects eNOS activity. To answer these
questions, we have cloned the bovine caveolin-1 cDNA and have investigated the eNOS-
caveolin-1 interaction in an in vitrobinding assay system using glutathione S-transferase
(GST)-caveolin-1 fusion proteins and baculovirus-expressed bovine eNOS. We have also …
plasmalemmal caveolae. It is not known, however, whether eNOS and caveolin-1 interact
directly or indirectly or whether the interaction affects eNOS activity. To answer these
questions, we have cloned the bovine caveolin-1 cDNA and have investigated the eNOS-
caveolin-1 interaction in an in vitrobinding assay system using glutathione S-transferase
(GST)-caveolin-1 fusion proteins and baculovirus-expressed bovine eNOS. We have also …