Cloning and expression of a complementary DNA encoding a high affinity human neurotensin receptor
N Vita, P Laurent, S Lefort, P Chalon, X Dumont… - FEBS …, 1993 - Wiley Online Library
N Vita, P Laurent, S Lefort, P Chalon, X Dumont, M Kaghad, D Gully, G Le Fur, P Ferrara…
FEBS letters, 1993•Wiley Online LibraryA human neurotensin receptor (hNTR) cDNA was cloned from the colonic adenocarcinoma
cell line HT29. The cloned cDNA encodes a putative peptide of 418 amino acids with 7
transmembrane domains. The amino acid sequence of the hNTR is 84% identical to the rat
NTR [Neuron, 4 (1990) 847‐854]. Transfection of this cDNA into COS cells results in the
expression of receptors with pharmacological properties similar to those found with HT29
cells. Northern blot analysis using the hNTR cDNA probe indicated a single transcript of 4 kb …
cell line HT29. The cloned cDNA encodes a putative peptide of 418 amino acids with 7
transmembrane domains. The amino acid sequence of the hNTR is 84% identical to the rat
NTR [Neuron, 4 (1990) 847‐854]. Transfection of this cDNA into COS cells results in the
expression of receptors with pharmacological properties similar to those found with HT29
cells. Northern blot analysis using the hNTR cDNA probe indicated a single transcript of 4 kb …
A human neurotensin receptor (hNTR) cDNA was cloned from the colonic adenocarcinoma cell line HT29. The cloned cDNA encodes a putative peptide of 418 amino acids with 7 transmembrane domains. The amino acid sequence of the hNTR is 84% identical to the rat NTR [Neuron, 4 (1990) 847‐854]. Transfection of this cDNA into COS cells results in the expression of receptors with pharmacological properties similar to those found with HT29 cells. Northern blot analysis using the hNTR cDNA probe indicated a single transcript of 4 kb in the brain, the small intestine and blood mononuclear cells.
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