Separation of neutrophils from blood in human and laboratory animals and comparison of the chemotaxis

T Sugawara, M Miyamoto, S Takayama… - Journal of pharmacological …, 1995 - Elsevier
We separated neutrophils from the peripheral blood of the human, monkey, dog, rabbit, rat,
hamster, and mouse and investigated their responses to several chemotactic factors to
evaluate the species-dependent difference in responsiveness. Heparinized blood was
obtained by venipuncture from human, monkey, dog, and rabbit. In the rat, hamster, and
mouse, the hetastarch exchange transfusion method was used to increase the neutrophil
recovery. After sedimentation of red blood cells, a leukocyte suspension was layered on 5 …