[2] Utilization of labeled Escherichia coli as phospholipase substrate
P Elsbach, J Weiss - Methods in enzymology, 1991 - Elsevier
Publisher Summary Escherichia coli marked with 14 C-labeled fatty acid have proved to be
convenient for the assay of deacylating phospholipases. They have been used in two ways:
as intact bacteria and after autoclaving. As is the case for phospholipids in the membranes
of most unperturbed cells, the phospholipids of intact E. coli are refractory to the action of
both endogenous and added deacylating phospholipases. This helps in studying the ability
of various agents to activate selectively or indiscriminately phospholipolysis by a broad …
convenient for the assay of deacylating phospholipases. They have been used in two ways:
as intact bacteria and after autoclaving. As is the case for phospholipids in the membranes
of most unperturbed cells, the phospholipids of intact E. coli are refractory to the action of
both endogenous and added deacylating phospholipases. This helps in studying the ability
of various agents to activate selectively or indiscriminately phospholipolysis by a broad …