Structural and functional properties of a phospholipase A2 purified from an inflammatory exudate
S Forst, J Weiss, P Elsbach, JM Maraganore… - Biochemistry, 1986 - ACS Publications
S Forst, J Weiss, P Elsbach, JM Maraganore, I Reardon, RL Heinrikson
Biochemistry, 1986•ACS PublicationsMaterials and Methods Collection of Ascitic Fluid. Sterile inflammatory peritoneal exudates
were collected 14-18 h after intraperitoneal injection 1 Abbreviations: BPI, bactericidal
permeability increasing protein; PLA2, phospholipase A2; PMN, polymorphonuclear
leukocyte; TFA, trifluoroacetic acid; HPLC, high-pressure liquid chromatography; Tris-HC1,
tris (hydroxymethyl) aminomethane hydrochloride; Gdn-HCl, guanidine hydrochloride;
EDTA, ethylenediaminetetraaceticacid; EGTA, ethylene glycol bis (/3-aminoethyl ether)-7V …
were collected 14-18 h after intraperitoneal injection 1 Abbreviations: BPI, bactericidal
permeability increasing protein; PLA2, phospholipase A2; PMN, polymorphonuclear
leukocyte; TFA, trifluoroacetic acid; HPLC, high-pressure liquid chromatography; Tris-HC1,
tris (hydroxymethyl) aminomethane hydrochloride; Gdn-HCl, guanidine hydrochloride;
EDTA, ethylenediaminetetraaceticacid; EGTA, ethylene glycol bis (/3-aminoethyl ether)-7V …
Materials and Methods
Collection of Ascitic Fluid. Sterile inflammatory peritoneal exudates were collected 14-18 h after intraperitoneal injection
1 Abbreviations: BPI, bactericidal permeability increasing protein; PLA2, phospholipase A2; PMN, polymorphonuclear leukocyte; TFA, trifluoroacetic acid; HPLC, high-pressure liquid chromatography; Tris-HC1, tris (hydroxymethyl) aminomethane hydrochloride; Gdn-HCl, guanidine hydrochloride; EDTA, ethylenediaminetetraaceticacid; EGTA, ethylene glycol bis (/3-aminoethyl ether)-7V, Ar, A'', Af'-tetraacetic acid.
ACS Publications