Membrane currents in the rabbit sinoatrial node cell as studied by the double microelectrode method
A Noma, H Irisawa - Pflügers Archiv, 1976 - Springer
A Noma, H Irisawa
Pflügers Archiv, 1976•SpringerWhen a strand of the rabbit sinoatrial node tissue was shortened by ligation, the spatial
decay of electrotonic potential decreased and the input impedance increased. In a piece of
the tissue 0.2–0.3 mm in diameter apparently uniform current spread was obtained. Action
potentials recorded from three different sites in this small piece occurred simultaneously and
were superimposable. In voltage clamp experiments using the double microelectrode
method, the membrane potential was usually held at− 30 to− 40 mV, where no net current …
decay of electrotonic potential decreased and the input impedance increased. In a piece of
the tissue 0.2–0.3 mm in diameter apparently uniform current spread was obtained. Action
potentials recorded from three different sites in this small piece occurred simultaneously and
were superimposable. In voltage clamp experiments using the double microelectrode
method, the membrane potential was usually held at− 30 to− 40 mV, where no net current …
Summary
When a strand of the rabbit sinoatrial node tissue was shortened by ligation, the spatial decay of electrotonic potential decreased and the input impedance increased. In a piece of the tissue 0.2–0.3 mm in diameter apparently uniform current spread was obtained. Action potentials recorded from three different sites in this small piece occurred simultaneously and were superimposable.
In voltage clamp experiments using the double microelectrode method, the membrane potential was usually held at −30 to −40 mV, where no net current flowed. When membrane potential was suddenly changed from the holding potential, the sign and the time course of the ionic current varied with membrane potential. Hyperpolarization gave an inward current which increased with time. Depolarization gave a transient inward current followed by sustained outward current, and repolarization gave an outward current tail which exponentially subsided with a time constant of 0.37 s.
The membrane time constant was 12.0 ms. When the specific membrane capacitance was assumed to be 1 μF/cm2, the specific membrane resistance at the resting potential was 12 kΩcm2. The peak of the transient inward current on depolarization was 1.3×10−5 A/cm2.
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