P-selectin (CD62) is a Ca²⁺-dependent lectin expressed on activated platelets and endothelium. Although P-selectin is known to function as a receptor for myeloid cells, previous studies indicated that P-selectin also bound to a subset of lymphocytes. Using a multi-color immunofluorescence assay we found that purified P-selectin bound to 12.2 ± 4.1 % of peripheral blood lymphocytes and that P-selectin could mediate adhesion of activated platelets to lymphocytes. A subpopulation of CD4⁺, CD8⁺, and CD16⁺ lymphocytes bound P-selectin. There was a marked preference for P-selectin binding to memory cells (CD45RO⁺) in both the CD4⁺ and CD8⁺ populations. Binding to all cell types was Ca²⁺-dependent and blocked by pretreatment of the cells with sialidase. These data suggest that P-selectin may play a role in the recruitment of specific lymphocyte populations to sites of inflammation.