Isolation, purification, and characterization of a lipoprotein containing apo B from the human aorta
HF Hoff, JW Gaubatz - Atherosclerosis, 1982 - Elsevier
LDL-like lipoproteins were extracted from a buffer-soluble fraction of homogenates of both
grossly normal intima and fatty-fibrous plaques from the human aorta. A low-speed
supernate of such homogenates was subjected to differential ultracentrifugation to isolate ad
1.006–1.063 density fraction which was further purified by gel filtration on agarose columns,
or by affinity chromatography of low-speed supernatants of aortic homogenates on
immunoabsorbents (anti-apo B). All recovered immunoreactivity for apo B as measured by …
grossly normal intima and fatty-fibrous plaques from the human aorta. A low-speed
supernate of such homogenates was subjected to differential ultracentrifugation to isolate ad
1.006–1.063 density fraction which was further purified by gel filtration on agarose columns,
or by affinity chromatography of low-speed supernatants of aortic homogenates on
immunoabsorbents (anti-apo B). All recovered immunoreactivity for apo B as measured by …