New Ki-67-equivalent murine monoclonal antibodies (MIB 1-3) generated against bacterially expressed parts of the Ki-67 cDNA containing three 62 base pair …
G Key, MH Becker, B Baron, M Duchrow… - … ; a journal of technical …, 1993 - europepmc.org
G Key, MH Becker, B Baron, M Duchrow, C Schlüter, HD Flad, J Gerdes
Laboratory investigation; a journal of technical methods and pathology, 1993•europepmc.orgBackground The monoclonal antibody Ki-67 reacts with a human nuclear cell proliferation-
associated antigen that is expressed in all cells that are not in G0. Recently, we could
demonstrate that Ki-67 detects a double band in Western blots of proliferating cells with
apparent molecular weights of 345 kilodaltons and 395 kilodaltons, respectively.
Furthermore, initial molecular biologic data favored the view that the epitope detected by Ki-
67 might be encoded by a repetitive 66 bp element.
associated antigen that is expressed in all cells that are not in G0. Recently, we could
demonstrate that Ki-67 detects a double band in Western blots of proliferating cells with
apparent molecular weights of 345 kilodaltons and 395 kilodaltons, respectively.
Furthermore, initial molecular biologic data favored the view that the epitope detected by Ki-
67 might be encoded by a repetitive 66 bp element.
Background
The monoclonal antibody Ki-67 reacts with a human nuclear cell proliferation-associated antigen that is expressed in all cells that are not in G0. Recently, we could demonstrate that Ki-67 detects a double band in Western blots of proliferating cells with apparent molecular weights of 345 kilodaltons and 395 kilodaltons, respectively. Furthermore, initial molecular biologic data favored the view that the epitope detected by Ki-67 might be encoded by a repetitive 66 bp element.
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