Molecular cloning and functional expression of the human gallbladder cholecystokinin A receptor
CD Ulrich, I Ferber, E Holicky, E Hadac, G Buell… - Biochemical and …, 1993 - Elsevier
CD Ulrich, I Ferber, E Holicky, E Hadac, G Buell, LJ Miller
Biochemical and biophysical research communications, 1993•ElsevierThrough binding to cholecystokinin (CCK) A receptors, CCK is an important physiologic
regulator of both gallbladder contraction and pancreatic enzyme secretion. In this work, we
have used a combination of hybridization screening of a cDNA library and polymerase chain
reaction to clone a 2.1 kb cDNA which encodes the human gallbladder CCK A receptor.
Nucleotide sequence analysis revealed an open reading frame encoding a 428 amino acid
protein, with seven putative transmembrane domains and a high degree of homology with …
regulator of both gallbladder contraction and pancreatic enzyme secretion. In this work, we
have used a combination of hybridization screening of a cDNA library and polymerase chain
reaction to clone a 2.1 kb cDNA which encodes the human gallbladder CCK A receptor.
Nucleotide sequence analysis revealed an open reading frame encoding a 428 amino acid
protein, with seven putative transmembrane domains and a high degree of homology with …
Abstract
Through binding to cholecystokinin (CCK) A receptors, CCK is an important physiologic regulator of both gallbladder contraction and pancreatic enzyme secretion. In this work, we have used a combination of hybridization screening of a cDNA library and polymerase chain reaction to clone a 2.1 kb cDNA which encodes the human gallbladder CCKA receptor. Nucleotide sequence analysis revealed an open reading frame encoding a 428 amino acid protein, with seven putative transmembrane domains and a high degree of homology with the rat CCKA receptor. COS cells transfected with this cDNA clone bound CCK-8 and L-364,718 with high affinities appropriate for the CCKA receptor, and exhibited a transient increase in intracellular calcium in response to CCK. This should provide an important resource for the analysis of the role of this receptor in human physiology and pathophysiology.
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