[HTML][HTML] Defining of the Minimal Domain of Protein 4.1 Involved in Spectrin-Actin Binding (∗)
PO Schischmanoff, R Winardi, DE Discher… - Journal of Biological …, 1995 - ASBMB
The spectrin-actin-binding domain of protein 4.1 is encoded by a 21-amino acid alternative
exon and a 59-amino acid constitutive exon. To characterize the minimal domain active for
interactions with spectrin and actin, we functionally characterized recombinant 4.1 peptides
containing the 21-amino acid cassette plus varying portions of the 59-amino acid cassette
(designated 21.10 to 21.59). Peptide 21.43 was shown fully functional in binary interactions
with spectrin (by cosedimentation and coimmunoprecipitation experiments) and in ternary …
exon and a 59-amino acid constitutive exon. To characterize the minimal domain active for
interactions with spectrin and actin, we functionally characterized recombinant 4.1 peptides
containing the 21-amino acid cassette plus varying portions of the 59-amino acid cassette
(designated 21.10 to 21.59). Peptide 21.43 was shown fully functional in binary interactions
with spectrin (by cosedimentation and coimmunoprecipitation experiments) and in ternary …