Thyroid hormone inhibition of human thyrotropin β-subunit gene expression is mediated by a cis-acting element located in the first exon
FE Wondisford, EA Farr, S Radovick… - Journal of Biological …, 1989 - ASBMB
Thyroid hormone regulation of the human thyrotropin β-subunit gene (TSHβ) was examined
in a human embryonal cell line (293). Transient expression studies were performed with
chimeric plasmids containing the reporter gene, chloramphenicol acetyltransferase.
Sequences in the first exon between+ 9 and+ 37 base pairs (bp) enhanced gene expression
from the human TSHβ promoter in the absence of thyroid hormone as well as mediated a
concentration-dependent triiodothyronine (LT 3) decrease in gene expression. Thyroid …
in a human embryonal cell line (293). Transient expression studies were performed with
chimeric plasmids containing the reporter gene, chloramphenicol acetyltransferase.
Sequences in the first exon between+ 9 and+ 37 base pairs (bp) enhanced gene expression
from the human TSHβ promoter in the absence of thyroid hormone as well as mediated a
concentration-dependent triiodothyronine (LT 3) decrease in gene expression. Thyroid …