The human 32-kDa stress protein induced by exposure to arsenite and cadmium ions is heme oxygenase
S Taketani, H Kohno, T Yoshinaga, R Tokunaga - FEBS letters, 1989 - Elsevier
S Taketani, H Kohno, T Yoshinaga, R Tokunaga
FEBS letters, 1989•ElsevierExposure of HeLa and HL60 cells to sodium arsenite or cadmium chloride led to marked
increases in cellular heme oxygenase activity. SDS-polyacrylamide gel clectrophoresis of
[35S] methionine-labeled cellular proteins indicated that these treatments also resulted in
the induction of a 32-kDa protein. Immunoblot analysis further showed that the 32-kDa
protein reacted with anti-bovine heme oxygenase antibodies. Treatment of the cells with
cobaltic chloride or heat induced neither the 32-kDa protein nor heme oxygenase activity. It …
increases in cellular heme oxygenase activity. SDS-polyacrylamide gel clectrophoresis of
[35S] methionine-labeled cellular proteins indicated that these treatments also resulted in
the induction of a 32-kDa protein. Immunoblot analysis further showed that the 32-kDa
protein reacted with anti-bovine heme oxygenase antibodies. Treatment of the cells with
cobaltic chloride or heat induced neither the 32-kDa protein nor heme oxygenase activity. It …
Abstract
Exposure of HeLa and HL60 cells to sodium arsenite or cadmium chloride led to marked increases in cellular heme oxygenase activity. SDS-polyacrylamide gel clectrophoresis of [35S]methionine-labeled cellular proteins indicated that these treatments also resulted in the induction of a 32-kDa protein. Immunoblot analysis further showed that the 32-kDa protein reacted with anti-bovine heme oxygenase antibodies. Treatment of the cells with cobaltic chloride or heat induced neither the 32-kDa protein nor heme oxygenase activity. It is concluded that the 32-kDa stress protein induced by arsenite and cadmium ions in these human cells is heme oxygenase.
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