Evidence of Haemophilus ducreyi adherence to and cytotoxin destruction of human epithelial cells
T Lagergård, M Purvén, A Frisk - Microbial pathogenesis, 1993 - Elsevier
The adherence of ten different Haemophilus ducreyi strains to cultured human epithelial
cells and the subsequent destruction of these cells was investigated in vitro using H Ep-2
and HeLa cells. Bacterial adherence was measured with two assays, one employing viable
bacteria and the other radiolabeled bacteria. In addition, the capacity of H. ducreyi to
invade/penetrate the H Ep-2 cells was examined. Differential interference contrast and
transmission electron microscopy techniques were also used. In both cell lines, all ten …
cells and the subsequent destruction of these cells was investigated in vitro using H Ep-2
and HeLa cells. Bacterial adherence was measured with two assays, one employing viable
bacteria and the other radiolabeled bacteria. In addition, the capacity of H. ducreyi to
invade/penetrate the H Ep-2 cells was examined. Differential interference contrast and
transmission electron microscopy techniques were also used. In both cell lines, all ten …