Systematic approach to the development of plasma amino acid analysis by high-performance liquid chromatography with ultraviolet detection with precolumn …
M Hariharan, S Naga, T VanNoord - Journal of Chromatography B …, 1993 - Elsevier
A reversed-phase liquid chromatographic method for the separation of 26
phenylthiocarbamyl derivatives of amino acids in human plasma in ca. 35 min. is described.
The method used a C 18 column (150× 4.6 mm ID, 3 μm) thermostatted at 41° C, and a
simple multistep linear gradient of two solvents. Solvent A was 0.05 M sodium acetate (pH
5.1)—acetonitrile (98: 2, v/v), and solvent B was water—acetonitrile (40: 60, v/v). A simple
and successful approach to the optimization of the conditions for the separation of the 26 …
phenylthiocarbamyl derivatives of amino acids in human plasma in ca. 35 min. is described.
The method used a C 18 column (150× 4.6 mm ID, 3 μm) thermostatted at 41° C, and a
simple multistep linear gradient of two solvents. Solvent A was 0.05 M sodium acetate (pH
5.1)—acetonitrile (98: 2, v/v), and solvent B was water—acetonitrile (40: 60, v/v). A simple
and successful approach to the optimization of the conditions for the separation of the 26 …