[HTML][HTML] Hyperacidification of cellubrevin endocytic compartments and defective endosomal recycling in cystic fibrosis respiratory epithelial cells
JF Poschet, J Skidmore, JC Boucher… - Journal of Biological …, 2002 - ASBMB
The cystic fibrosis transmembrane conductance regulator (CFTR), which is aberrant in
patients with cystic fibrosis, normally functions both as a chloride channel and as a
pleiotropic regulator of other ion transporters. Here we show, by ratiometric imaging with
luminally exposed pH-sensitive green fluorescent protein, that CFTR affects the pH of
cellubrevin-labeled endosomal organelles resulting in hyperacidification of these
compartments in cystic fibrosis lung epithelial cells. The excessive acidification of …
patients with cystic fibrosis, normally functions both as a chloride channel and as a
pleiotropic regulator of other ion transporters. Here we show, by ratiometric imaging with
luminally exposed pH-sensitive green fluorescent protein, that CFTR affects the pH of
cellubrevin-labeled endosomal organelles resulting in hyperacidification of these
compartments in cystic fibrosis lung epithelial cells. The excessive acidification of …