Pseudomonas exotoxin: chimeric toxins
I Pastan, D FitzGerald - Journal of Biological Chemistry, 1989 - ASBMB
Pseudomonas exotoxin binds to and enters cells by receptor-mediated endocytosis. Within
the cell it requires exposure to low pH to enable it to translocate to the cell cytoplasm where
it inhibits protein synthesis by ADP-ribosylating elongation factor 2. The toxin has three main
structural domains whose functions are: Ia, cell binding; II, translocation; and III, ADP-
ribosylation. Key amino acids have been identified within each domain that are required for
the function of the toxin. Chimeric toxins were made originally by using chemical cross …
the cell it requires exposure to low pH to enable it to translocate to the cell cytoplasm where
it inhibits protein synthesis by ADP-ribosylating elongation factor 2. The toxin has three main
structural domains whose functions are: Ia, cell binding; II, translocation; and III, ADP-
ribosylation. Key amino acids have been identified within each domain that are required for
the function of the toxin. Chimeric toxins were made originally by using chemical cross …