Blot hybridisation analysis of genomic DNA.
S Vandenplas, I Wiid, A Grobler-Rabie… - Journal of Medical …, 1984 - jmg.bmj.com
S Vandenplas, I Wiid, A Grobler-Rabie, K Brebner, M Ricketts, G Wållis, A Bester, C Boyd…
Journal of Medical Genetics, 1984•jmg.bmj.comRestriction endonuclease analysis of specific gene sequences is proving to be a valuable
technique for characterisation and diagnosis of inherited disorders. This paper describes
detailed protocols for isolation, restriction, and blot hybridisation of genomic DNA. Problems
and alternatives in the procedure are discussed and a troubleshooting guide has been
provided to help rectify faults.
technique for characterisation and diagnosis of inherited disorders. This paper describes
detailed protocols for isolation, restriction, and blot hybridisation of genomic DNA. Problems
and alternatives in the procedure are discussed and a troubleshooting guide has been
provided to help rectify faults.
Restriction endonuclease analysis of specific gene sequences is proving to be a valuable technique for characterisation and diagnosis of inherited disorders. This paper describes detailed protocols for isolation, restriction, and blot hybridisation of genomic DNA. Problems and alternatives in the procedure are discussed and a troubleshooting guide has been provided to help rectify faults.
jmg.bmj.com