Published March 1, 2017 - More info
MicroRNAs (miRNAs) influence many biological processes, including cancer. They do so by posttranscriptionally repressing target mRNAs to which they have sequence complementarity. Although it has been postulated that miRNAs can regulate other miRNAs, this has never been shown experimentally to our knowledge. Here, we demonstrate that miR-107 negatively regulates the tumor suppressor miRNA let-7 via a direct interaction. miR-107 was found to be highly expressed in malignant tissue from patients with advanced breast cancer, and its expression was inversely correlated with let-7 expression in tumors and in cancer cell lines. Ectopic expression of miR-107 in human cancer cell lines led to destabilization of mature let-7, increased expression of let-7 targets, and increased malignant phenotypes. In contrast, depletion of endogenous miR-107 dramatically increased the stability of mature let-7 and led to downregulation of let-7 targets. Accordingly, miR-107 expression increased the tumorigenic and metastatic potential of a human breast cancer cell line in mice via inhibition of let-7 and upregulation of let-7 targets. By mutating individual sites within miR-107 and let-7, we found that miR-107 directly interacts with let-7 and that the internal loop of the let-7/miR-107 duplex is critical for repression of let-7 expression. Altogether, we have identified an oncogenic role for miR-107 and provide evidence of a transregulational interaction among miRNAs in human cancer development.
Pai-Sheng Chen, Jen-Liang Su, Shih-Ting Cha, Woan-Yuh Tarn, Ming-Yang Wang, Hsing-Chih Hsu, Ming-Tsan Lin, Chia-Yu Chu, Kuo-Tai Hua, Chiung-Nien Chen, Tsang-Chih Kuo, King-Jen Chang, Michael Hsiao, Yi-Wen Chang, Jin-Shing Chen, Pan-Chyr Yang, Min-Liang Kuo
Original citation: J Clin Invest. 2011;121(9):3442–3455. https://doi.org/10.1172/JCI45390
Citation for this corrigendum: J Clin Invest. 2017;127(3):1116. https://doi.org/10.1172/JCI92099
The authors recently became aware of errors in the assembly of Figure 3A that resulted in incorrect images for the U6 Northern blot for the lung cancer and breast cancer cell lines. The authors were able to provide the original source data with the correct images. The corrected figure panel is below.
The authors regret the errors.
See the related article at miR-107 promotes tumor progression by targeting the let-7 microRNA in mice and humans.