Donor T cells that respond to host alloantigens following allogeneic bone marrow transplantation (BMT) induce graft-versus-host (GVH) responses, but their molecular landscape is not well understood. MicroRNAs (miRNAs) regulate gene (mRNA) expression and fine-tune the molecular responses of T cells. We stimulated naive T cells with either allogeneic or nonspecific stimuli and used argonaute cross-linked immunoprecipitation (CLIP) with subsequent ChIP microarray analyses to profile miR responses and their direct mRNA targets. We identified a unique expression pattern of miRs and mRNAs following the allostimulation of T cells and a high correlation between the expression of the identified miRs and a reduction of their mRNA targets. miRs and mRNAs that were predicted to be differentially regulated in allogeneic T cells compared with nonspecifically stimulated T cells were validated in vitro. These analyses identified wings apart-like homolog (
Yaping Sun, Isao Tawara, Meng Zhao, Zhaohui S. Qin, Tomomi Toubai, Nathan Mathewson, Hiroya Tamaki, Evelyn Nieves, Arul M. Chinnaiyan, Pavan Reddy
AGO-CLIP-ChIP procedures and mRNA CLIP-ChIP profiles of Allo T versus CD3/28 T cells.