TY - JOUR AU - Wang, Xiaoli AU - Prakash, Sonam AU - Lu, Min AU - Tripodi, Joseph AU - Ye, Fei AU - Najfeld, Vesna AU - Li, Yan AU - Schwartz, Myron AU - Weinberg, Rona AU - Roda, Paul AU - Orazi, Attilio AU - Hoffman, Ronald T1 - Spleens of myelofibrosis patients contain malignant hematopoietic stem cells PY - 2012/11/01/ AB - Cancer stem cell behavior is thought to be largely determined by intrinsic properties and by regulatory signals provided by the microenvironment. Myelofibrosis (MF) is characterized by hematopoiesis occurring not only in the marrow but also in extramedullary sites such as the spleen. In order to study the effects of these different microenvironments on primitive malignant hematopoietic cells, we phenotypically and functionally characterized splenic and peripheral blood (PB) MF CD34+ cells from patients with MF. MF spleens contained greater numbers of malignant primitive HPCs than PB. Transplantation of PB MF CD34+ cells into immunodeficient (NOD/SCID/IL2RĪ³null) mice resulted in a limited degree of donor cell chimerism and a differentiation program skewed toward myeloid lineages. By contrast, transplanted splenic MF CD34+ cells achieved a higher level of chimerism and generated both myeloid and lymphoid cells that contained molecular or cytogenetic abnormalities indicating their malignant nature. Only splenic MF CD34+ cells were able to sustain hematopoiesis for prolonged periods (9 months) and were able to engraft secondary recipients. These data document the existence of MF stem cells (MF-SCs) that reside in the spleens of MF patients and demonstrate that these MF-SCs retain a differentiation program identical to that of normal hematopoietic stem cells. JF - The Journal of Clinical Investigation JA - J Clin Invest SN - 0021-9738 DO - 10.1172/JCI64397 VL - 122 IS - 11 UR - https://doi.org/10.1172/JCI64397 SP - 3888 EP - 3899 PB - The American Society for Clinical Investigation ER -