TY - JOUR AU - Matsumoto, Shingo AU - Hyodo, Fuminori AU - Subramanian, Sankaran AU - Devasahayam, Nallathamby AU - Munasinghe, Jeeva AU - Hyodo, Emi AU - Gadisetti, Chandramouli AU - Cook, John A. AU - Mitchell, James B. AU - Krishna, Murali C. T1 - Low-field paramagnetic resonance imaging of tumor oxygenation and glycolytic activity in mice PY - 2008/05/01/ AB - A priori knowledge of spatial and temporal changes in partial pressure of oxygen (oxygenation; pO2) in solid tumors, a key prognostic factor in cancer treatment outcome, could greatly improve treatment planning in radiotherapy and chemotherapy. Pulsed electron paramagnetic resonance imaging (EPRI) provides quantitative 3D maps of tissue pO2 in living objects. In this study, we implemented an EPRI set-up that could acquire pO2 maps in almost real time for 2D and in minutes for 3D. We also designed a combined EPRI and MRI system that enabled generation of pO2 maps with anatomic guidance. Using EPRI and an air/carbogen (95% O2 plus 5% CO2) breathing cycle, we visualized perfusion-limited hypoxia in murine tumors. The relationship between tumor blood perfusion and pO2 status was examined, and it was found that significant hypoxia existed even in regions that exhibited blood flow. In addition, high levels of lactate were identified even in normoxic tumor regions, suggesting the predominance of aerobic glycolysis in murine tumors. This report presents a rapid, noninvasive method to obtain quantitative maps of pO2 in tumors, reported with anatomy, with precision. In addition, this method may also be useful for studying the relationship between pO2 status and tumor-specific phenotypes such as aerobic glycolysis. JF - The Journal of Clinical Investigation JA - J Clin Invest SN - 0021-9738 DO - 10.1172/JCI34928 VL - 118 IS - 5 UR - https://doi.org/10.1172/JCI34928 SP - 1965 EP - 1973 PB - The American Society for Clinical Investigation ER -