Representative plots of (A) total NK cells and (B) CD56^bright and CD56^dim subsets in HCs and patients with DADA2. (C–F and I) Ex vivo percentage or median fluorescence intensity (MFI). (G) MFI of CD107a on isolated NK cells after 20 hours of culturing with or without K562 cells. (H) Percentage of dead K562 cells, corrected for background apoptosis and CD107a MFI on NK cells after 4 hours of coculturing. Orange dots refer to patient 8 and HC 8, where the assay used a lower effector/target ratio due to sample limitations (2.4:1 vs. 4:1). Each dot represents an individual; bars show the median with the IQR. *P < 0.05, **P < 0.01, and ****P < 0.0001, by 2-tailed, unpaired t test or Mann-Whitney U test (the exact test used is indicated in Supplemental Methods) (C–F, H, and I), or 1-way ANOVA with Šídák’s test (G). The complete gating strategy is shown in Supplemental Figure 1.