Advertisement

ResearchIn-Press PreviewAutoimmunityImmunology Open Access | 10.1172/JCI195987

Immobilized IgG-containing immune complexes require platelets to recruit neutrophils during inflammation

Marie Bellio,1 Isabelle Allaeys,1 Etienne Doré,1 Myriam Vaillancourt,1 Tania Lévesque,1 Mélina Monteil,1 Nicolas Vallières,1 Philippe Desaulniers,1 Nicolas Bertrand,2 Valance A. Washington,3 Yotis Senis,4 Steve Lacroix,1 Paul Fortin,1 Clémence Belleannée,1 and Eric Boilard1

1Faculté de Médecine de l’Université Laval, Université Laval, Québec, Canada

2Axe Endocrinologie et Néphrologie du Centre de recherche du Centre hospitalier universitaire de Québec-Université Laval, Québec, Canada

3Department of Biological Sciences, Oakland University, Rochester, United States of America

4Centre for Cardiovascualr and Nutrition Research, Institut National de la Santé Intergénérationanelle-Université Laval, Faculty of Medicine, Aix-Marseille University, Québec, France

Find articles by Bellio, M. in: PubMed | Google Scholar

1Faculté de Médecine de l’Université Laval, Université Laval, Québec, Canada

2Axe Endocrinologie et Néphrologie du Centre de recherche du Centre hospitalier universitaire de Québec-Université Laval, Québec, Canada

3Department of Biological Sciences, Oakland University, Rochester, United States of America

4Centre for Cardiovascualr and Nutrition Research, Institut National de la Santé Intergénérationanelle-Université Laval, Faculty of Medicine, Aix-Marseille University, Québec, France

Find articles by Allaeys, I. in: PubMed | Google Scholar |

1Faculté de Médecine de l’Université Laval, Université Laval, Québec, Canada

2Axe Endocrinologie et Néphrologie du Centre de recherche du Centre hospitalier universitaire de Québec-Université Laval, Québec, Canada

3Department of Biological Sciences, Oakland University, Rochester, United States of America

4Centre for Cardiovascualr and Nutrition Research, Institut National de la Santé Intergénérationanelle-Université Laval, Faculty of Medicine, Aix-Marseille University, Québec, France

Find articles by Doré, E. in: PubMed | Google Scholar |

1Faculté de Médecine de l’Université Laval, Université Laval, Québec, Canada

2Axe Endocrinologie et Néphrologie du Centre de recherche du Centre hospitalier universitaire de Québec-Université Laval, Québec, Canada

3Department of Biological Sciences, Oakland University, Rochester, United States of America

4Centre for Cardiovascualr and Nutrition Research, Institut National de la Santé Intergénérationanelle-Université Laval, Faculty of Medicine, Aix-Marseille University, Québec, France

Find articles by Vaillancourt, M. in: PubMed | Google Scholar

1Faculté de Médecine de l’Université Laval, Université Laval, Québec, Canada

2Axe Endocrinologie et Néphrologie du Centre de recherche du Centre hospitalier universitaire de Québec-Université Laval, Québec, Canada

3Department of Biological Sciences, Oakland University, Rochester, United States of America

4Centre for Cardiovascualr and Nutrition Research, Institut National de la Santé Intergénérationanelle-Université Laval, Faculty of Medicine, Aix-Marseille University, Québec, France

Find articles by Lévesque, T. in: PubMed | Google Scholar

1Faculté de Médecine de l’Université Laval, Université Laval, Québec, Canada

2Axe Endocrinologie et Néphrologie du Centre de recherche du Centre hospitalier universitaire de Québec-Université Laval, Québec, Canada

3Department of Biological Sciences, Oakland University, Rochester, United States of America

4Centre for Cardiovascualr and Nutrition Research, Institut National de la Santé Intergénérationanelle-Université Laval, Faculty of Medicine, Aix-Marseille University, Québec, France

Find articles by Monteil, M. in: PubMed | Google Scholar

1Faculté de Médecine de l’Université Laval, Université Laval, Québec, Canada

2Axe Endocrinologie et Néphrologie du Centre de recherche du Centre hospitalier universitaire de Québec-Université Laval, Québec, Canada

3Department of Biological Sciences, Oakland University, Rochester, United States of America

4Centre for Cardiovascualr and Nutrition Research, Institut National de la Santé Intergénérationanelle-Université Laval, Faculty of Medicine, Aix-Marseille University, Québec, France

Find articles by Vallières, N. in: PubMed | Google Scholar

1Faculté de Médecine de l’Université Laval, Université Laval, Québec, Canada

2Axe Endocrinologie et Néphrologie du Centre de recherche du Centre hospitalier universitaire de Québec-Université Laval, Québec, Canada

3Department of Biological Sciences, Oakland University, Rochester, United States of America

4Centre for Cardiovascualr and Nutrition Research, Institut National de la Santé Intergénérationanelle-Université Laval, Faculty of Medicine, Aix-Marseille University, Québec, France

Find articles by Desaulniers, P. in: PubMed | Google Scholar

1Faculté de Médecine de l’Université Laval, Université Laval, Québec, Canada

2Axe Endocrinologie et Néphrologie du Centre de recherche du Centre hospitalier universitaire de Québec-Université Laval, Québec, Canada

3Department of Biological Sciences, Oakland University, Rochester, United States of America

4Centre for Cardiovascualr and Nutrition Research, Institut National de la Santé Intergénérationanelle-Université Laval, Faculty of Medicine, Aix-Marseille University, Québec, France

Find articles by Bertrand, N. in: PubMed | Google Scholar

1Faculté de Médecine de l’Université Laval, Université Laval, Québec, Canada

2Axe Endocrinologie et Néphrologie du Centre de recherche du Centre hospitalier universitaire de Québec-Université Laval, Québec, Canada

3Department of Biological Sciences, Oakland University, Rochester, United States of America

4Centre for Cardiovascualr and Nutrition Research, Institut National de la Santé Intergénérationanelle-Université Laval, Faculty of Medicine, Aix-Marseille University, Québec, France

Find articles by Washington, V. in: PubMed | Google Scholar

1Faculté de Médecine de l’Université Laval, Université Laval, Québec, Canada

2Axe Endocrinologie et Néphrologie du Centre de recherche du Centre hospitalier universitaire de Québec-Université Laval, Québec, Canada

3Department of Biological Sciences, Oakland University, Rochester, United States of America

4Centre for Cardiovascualr and Nutrition Research, Institut National de la Santé Intergénérationanelle-Université Laval, Faculty of Medicine, Aix-Marseille University, Québec, France

Find articles by Senis, Y. in: PubMed | Google Scholar

1Faculté de Médecine de l’Université Laval, Université Laval, Québec, Canada

2Axe Endocrinologie et Néphrologie du Centre de recherche du Centre hospitalier universitaire de Québec-Université Laval, Québec, Canada

3Department of Biological Sciences, Oakland University, Rochester, United States of America

4Centre for Cardiovascualr and Nutrition Research, Institut National de la Santé Intergénérationanelle-Université Laval, Faculty of Medicine, Aix-Marseille University, Québec, France

Find articles by Lacroix, S. in: PubMed | Google Scholar

1Faculté de Médecine de l’Université Laval, Université Laval, Québec, Canada

2Axe Endocrinologie et Néphrologie du Centre de recherche du Centre hospitalier universitaire de Québec-Université Laval, Québec, Canada

3Department of Biological Sciences, Oakland University, Rochester, United States of America

4Centre for Cardiovascualr and Nutrition Research, Institut National de la Santé Intergénérationanelle-Université Laval, Faculty of Medicine, Aix-Marseille University, Québec, France

Find articles by Fortin, P. in: PubMed | Google Scholar

1Faculté de Médecine de l’Université Laval, Université Laval, Québec, Canada

2Axe Endocrinologie et Néphrologie du Centre de recherche du Centre hospitalier universitaire de Québec-Université Laval, Québec, Canada

3Department of Biological Sciences, Oakland University, Rochester, United States of America

4Centre for Cardiovascualr and Nutrition Research, Institut National de la Santé Intergénérationanelle-Université Laval, Faculty of Medicine, Aix-Marseille University, Québec, France

Find articles by Belleannée, C. in: PubMed | Google Scholar

1Faculté de Médecine de l’Université Laval, Université Laval, Québec, Canada

2Axe Endocrinologie et Néphrologie du Centre de recherche du Centre hospitalier universitaire de Québec-Université Laval, Québec, Canada

3Department of Biological Sciences, Oakland University, Rochester, United States of America

4Centre for Cardiovascualr and Nutrition Research, Institut National de la Santé Intergénérationanelle-Université Laval, Faculty of Medicine, Aix-Marseille University, Québec, France

Find articles by Boilard, E. in: PubMed | Google Scholar |

Published December 2, 2025 - More info

J Clin Invest. https://doi.org/10.1172/JCI195987.
Copyright © 2025, Bellio et al. This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Published December 2, 2025 - Version history
View PDF
Abstract

During vascular injury, platelets are essential for halting bleeding and recruiting neutrophils to prevent microbial invasion. However, in antibody-mediated autoimmune diseases occurring without vascular damage, neutrophils infiltrate tissues and contribute to pathology. Here, we investigated whether the dependence of neutrophils on platelets is conserved in the context of antibody-driven inflammation. Using human cells from individuals with rheumatoid arthritis and a microfluidic system mimicking physiological shear over IgG-containing immune complexes, we demonstrate that despite expressing Fc receptors, neutrophils require platelets to stably adhere to immune complexes under flow. Platelet FcγRIIA binding was critical for resisting shear stress, while neutrophils used FcγRIIA and FcγRIIIB for immune complex recognition. Platelet P-selectin binding to neutrophil PSGL-1 was essential for recruitment, whereas Mac-1 was dispensable. In a mouse model of autoantibody-mediated arthritis, intravital imaging confirmed that neutrophil recruitment critically relies on PSGL-1. Importantly, expression of FcyRIIA aggravated arthritis, and blockade of PSGL-1 in these mice, but not of Mac-1, abrogated both the platelet and neutrophil interactions and disease. These findings identify key molecular interactions in platelet–neutrophil cooperation and reveal that platelets are essential enablers of FcR-mediated neutrophil adhesion in antibody-driven inflammation.  

Graphical Abstract
graphical abstract
Supplemental material

View

View Movie 1A

View Movie 1B

View Movie 2-PBS

View Movie 3_isotype

View Movie 4_anti-PSGL-1

View Movie 5

Version history
  • Version 1 (December 2, 2025): In-Press Preview
Advertisement
Advertisement