FDC function is impaired in animals treated with LTβR-Ig, and FDCs recover function after 3 months. Spleens from untreated (a and d), treated (8 mg/kg LTβR-Ig) (b and e), and recovery animals (8 mg/kg treatment followed by 3-month recovery) (c and f) injected with PAP were stained with anti-CD20 (green) to identify B cell follicles and TSA-rhodamine (red) to identify PAP networks. PAP networks are visible within CD20+ B cell follicles (arrows). (a, b, c) 100×. (d, e, f) ×50. Spleen sections were analyzed for the number of PAP networks in a given area of tissue both after treatment and after recovery periods. Doses were combined for each time point (g).