TIMP-1 DNA inhibits angiogenic scoring and HSK lesion severity. Groups of animals were treated twice with 100 μg of TIMP-1 DNA and vector DNA intraocularly 6 and 3 days before virus infection and were infected with 106 PFU of HSV-1 RE on their scarified corneas 3 days after a second treatment of DNAs. The mice were examined clinically by slit-lamp microscopy, and the severity of lesions and the extent of angiogenesis were scored as described in Methods. Data are compiled from three independent experiments with seven individual mice per group. Significant differences (P ≤ 0.05) between TIMP-1 DNA– and vector DNA–treated mice are indicated by an asterisk (angiogenic scoring) and a double asterisk (severity). Such differences were present at day 5 and beyond (angiogenic scoring) and at day 10 and beyond (HSK lesion severity).