Phagocytic but not endocytic uptake of antibody-complexed antigen requires activating FcγRs on DCs. Immature BMDCs were incubated for 30 minutes with either OVA-containing ICs for endocytosis or antibody-opsonized SRBCs for phagocytosis. Uptake of soluble OVA or anti-OVA alone was negligible (data not shown), while uptake of OVA/ICs was dramatically enhanced for both WT and FcγRγ–/– BMDCs, indicating that inhibitory FcRs are capable of efficiently endocytosing OVA/ICs. In contrast, phagocytic uptake of antibody-opsonized SRBCs was seen in WT but not in FcγRγ–/– BMDCs.