Expression of CLA by in vitro restimulated herpesvirus-specific CD8+ T cells. For HSV-2–specific T cells, unique subject ID numbers are indicated below the HSV-2 antigens. (a) PBMCs from an HLA B7-bearing, HSV-2–infected (panel 1) or two different HLA A2-bearing subjects (panels 2 and 3) stimulated for 13 days with HSV-2 VP22 a.a. 49–57, HSV-2 ICP0 a.a. 743–751, or HSV-2 VP 13/14 a.a. 551–559, respectively. Subjects are described in Table 1. PBMC from two HLA A2-bearing subjects were stimulated with EBV BMLF-1 a.a. 280–288 (panels 4 and 5). Dot plots display binding of the relevant tetramers and anti-CD8α. Quadrant lines are cutoffs for CD8α-high cells and tetramer binding. The percentages of CD8α-high cells that are tetramer-positive are indicated. (b) Expression of CLA by CD8α-high lymphocytes that stain with HSV-2 or EBV tetramers. (c) Expression of CLA by CD8α-high lymphocytes that do not stain with tetramer. Percentages of CLA-positive cells are indicated.