@article{10.1172/JCI152379, author = {Anthony T. Tan AND Joey M.E. Lim AND Nina Le Bert AND Kamini Kunasegaran AND Adeline Chia AND Martin D.C. Qui AND Nicole Tan AND Wan Ni Chia AND Ruklanthi de Alwis AND Ding Ying AND Jean X.Y. Sim AND Eng Eong Ooi AND Lin-Fa Wang AND Mark I-Cheng Chen AND Barnaby E. Young AND Li Yang Hsu AND Jenny G.H. Low AND David C. Lye AND Antonio Bertoletti}, journal = {The Journal of Clinical Investigation}, publisher = {The American Society for Clinical Investigation}, title = {Rapid measurement of SARS-CoV-2 spike T cells in whole blood from vaccinated and naturally infected individuals}, year = {2021}, month = {9}, volume = {131}, url = {https://www.jci.org/articles/view/152379}, abstract = {Defining the correlates of protection necessary to manage the COVID-19 pandemic requires the analysis of both antibody and T cell parameters, but the complexity of traditional tests limits virus-specific T cell measurements. We tested the sensitivity and performance of a simple and rapid SARS-CoV-2 spike protein–specific T cell test based on the stimulation of whole blood with peptides covering the SARS-CoV-2 spike protein, followed by cytokine (IFN-γ, IL-2) measurement in different cohorts including BNT162b2-vaccinated individuals (n = 112), convalescent asymptomatic and symptomatic COVID-19 patients (n = 130), and SARS-CoV-1–convalescent individuals (n = 12). The sensitivity of this rapid test is comparable to that of traditional methods of T cell analysis (ELISPOT, activation-induced marker). Using this test, we observed a similar mean magnitude of T cell responses between the vaccinees and SARS-CoV-2 convalescents 3 months after vaccination or virus priming. However, a wide heterogeneity of the magnitude of spike-specific T cell responses characterized the individual responses, irrespective of the time of analysis. The magnitude of these spike-specific T cell responses cannot be predicted from the neutralizing antibody levels. Hence, both humoral and cellular spike–specific immunity should be tested after vaccination to define the correlates of protection necessary to evaluate current vaccine strategies.}, number = {17}, doi = {10.1172/JCI152379}, url = {https://doi.org/10.1172/JCI152379}, }