Immunofluorescence staining of fibrin/fibrinogen and sheep IgG of wild-type and Gas6^–/– mice. (a) Immunohistological staining for fibrin/fibrinogen in wild-type and Gas6^–/– mice sacrificed on day 21. Magnification, ×200. (b) Quantitative assessment of fibrin and fibrinogen staining of wild-type and Gas6^–/– mice. Each group contained eight mice, and 40 glomeruli per mouse were evaluated in a blinded fashion. Data are expressed as mean ± SD. ***P < 0.001. (c) Representative immunostaining for sheep IgG in mice sacrificed on day 7. Magnification, ×40. Filled bars represent Gas6^–/– mice, open bars represent wild-type mice. (d) Circulating titers of mouse anti-sheep IgG 21 days after NTS injection were measured by ELISA in both wild-type and Gas6^–/– mice. Serum dilutions were 1:1,000. Each group consisted of six mice, and data are expressed as mean ± SD. NS, not significant.