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Burst-like control of lipolysis by the sympathetic nervous system in vivo
Katrin Hücking, … , Martin Ellmerer, Richard N. Bergman
Katrin Hücking, … , Martin Ellmerer, Richard N. Bergman
Published January 15, 2003
Citation Information: J Clin Invest. 2003;111(2):257-264. https://doi.org/10.1172/JCI14466.
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Article Endocrinology

Burst-like control of lipolysis by the sympathetic nervous system in vivo

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Abstract

Rapid oscillations of visceral lipolysis have been reported. To examine the putative role of the CNS in oscillatory lipolysis, we tested the effects of β3-blockade on pulsatile release of FFAs. Arterial blood samples were drawn at 1-minute intervals for 120 minutes from fasted, conscious dogs (n = 7) during the infusion of saline or bupranolol (1.5 μg/kg/min), a high-affinity β3-blocker. FFA and glycerol time series were analyzed and deconvolution analysis was applied to estimate the rate of FFA release. During saline infusion FFAs and glycerol oscillated in phase at about eight pulses/hour. Deconvolution analysis showed bursts of lipolysis (nine pulses/hour) with time-dependent variation in burst frequency. Bupranolol completely removed rapid FFA and glycerol oscillations. Despite removal of lipolytic bursts, plasma FFAs (0.31 mM) and glycerol (0.06 mM) were not totally suppressed and deconvolution analysis revealed persistent non-oscillatory lipolysis (0.064 mM/min). These results show that lipolysis in the fasting state consists of an oscillatory component, which appears to be entirely dependent upon sympathetic innervation of the adipose tissue, and a non-oscillatory, constitutive component, which persists despite β3-blockade. The extinction of lipid fuel bursts by β3-blockade implies a role for the CNS in the maintenance of cyclic provision of lipid fuels.

Authors

Katrin Hücking, Marianthe Hamilton-Wessler, Martin Ellmerer, Richard N. Bergman

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Figure 1

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Reconstruction of infused FFA pulses with deconvolution analysis. (a) Up...
Reconstruction of infused FFA pulses with deconvolution analysis. (a) Upper graph shows the measured FFA concentration (filled circles) with best fit to concentration data (solid line). The profile of FFA release calculated by deconvolution analysis (middle graph) is in accordance with the infused FFA profile (five 90-second pulses with 0.5 mmol octanoate per pulse, bottom graph). (b) A strong correlation exists between the predicted FFA pulses and actually infused octanoate pulses.

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ISSN: 0021-9738 (print), 1558-8238 (online)

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