Phoebe Carter, Ulrike Schnell, Christopher Chaney, Betty Tong, Xinchao Pan, Jianhua Ye, Glenda Mernaugh, Jennifer L. Cotton, Vitaly Margulis, Junhao Mao, Roy Zent, Bret M. Evers, Payal Kapur, Thomas J. Carroll
(A) Gene set enrichment analysis (GSEA) on Slc34a1CreERT2 Lats1/2c/c kidneys 4 months after tamoxifen. (B–D) Immunofluorescence staining on section of KspCreERT2 Lats1c/c Lats2c/c RosaYFP kidney 1 week after tamoxifen. (B) Green, E-cadherin; red, YFP; blue, DAPI; white, cytokeratin. Mutant, YFP-positive cells display loss of E-cadherin (C and D, arrows), while WT, YFP-negative tubules express E-cadherin (C and D, asterisks). C and D are single-channel images of YFP and E-cadherin, respectively, corresponding to the box in B. (E–G) Immunofluorescence staining on sections of KspCreERT2 Lats1c/c Lats2c/c RosaYFP kidneys 1 week, 1 month, and 8 months after tamoxifen. Green, E-cadherin; red, vimentin; blue, DAPI; white, YFP. (H–M) Immunofluorescence staining on section of KspCreERT2 Lats1c/c Lats2c/c RosaYFP kidney 1 week after tamoxifen. (H–J) Green, laminin; red, YFP; blue, DAPI. WT, YFP-negative tubules display intact basal lamina (asterisks), while surrounding mutant, YFP-positive cells exhibit disorganized basal lamina. I and J are single-channel images of H showing YFP and laminin, respectively. (K–M) Green, aPKC; red, YFP; blue, DAPI. WT, YFP-negative tubules show apical localization of aPKC (K–M, asterisks), which is not maintained in surrounding mutant, YFP-positive tubules. L and M are single-channel images of G showing YFP and aPKC, respectively. Experiments shown in B–M were conducted on 3 different mutants. Scale bars: (B–M) 100 μm.