WT and Sash mice were injected with CFSE 4 hours before s.c. FP injection of 1 × 10⁵ pfu DENV or saline. (A) FPs and (B) DLNs were collected 24 hours after infection, and CFSE⁺ and CFSE^– γδ T cells were enumerated by flow cytometry. Sash mice showed significantly reduced numbers of both CFSE⁺ and CFSE^– γδ T cells in FPs compared with WT mice. Representative histograms showing CFSE detection in CD4⁺, CD8⁺, and γδ T cell subsets in the DLNs of (C) uninfected and (D) infected WT mice. γδ T cells but not CD4⁺ or CD8⁺ T cells migrated to the DLNs from the site of infection. Multiple peaks in the histogram indicate proliferation of γδ T cells. Recruitment of T cells in the (E) FPs and (F) DLNs upon infection in WT but not Sash mice was confirmed by injecting CFSE-labeled splenocytes 24 hours prior to infection and analyzing the CFSE⁺ T cell numbers in FPs and DLNs 24 hours after infection. (G–I) Single-cell suspensions of LN cells, collected 24 hours and 48 hours after infection, were treated with monensin for 6 hours and then stained for the surface markers CD3, CD4, CD8, and γδ TCR and intracellularly for IFN-γ. Total numbers of IFN-γ–producing cells in WT, Sash, and Sash-R mice were subtyped on the basis of (G) γδ, (H) CD8⁺, and (I) CD4⁺ T cells. Data represent the mean ± SEM. *P < 0.05, **P < 0.01, and ****P < 0.0001, by 2-way ANOVA with Sidak’s multiple comparisons test. n = 3–6 mice per group.