Advertisement

Research Article Free access | 10.1172/JCI117580

Induction of manganese superoxide dismutase in rat cardiac myocytes increases tolerance to hypoxia 24 hours after preconditioning.

N Yamashita, M Nishida, S Hoshida, T Kuzuya, M Hori, N Taniguchi, T Kamada, and M Tada

First Department of Medicine, Osaka University Medical School, Japan.

Find articles by Yamashita, N. in: PubMed | Google Scholar

First Department of Medicine, Osaka University Medical School, Japan.

Find articles by Nishida, M. in: PubMed | Google Scholar

First Department of Medicine, Osaka University Medical School, Japan.

Find articles by Hoshida, S. in: PubMed | Google Scholar

First Department of Medicine, Osaka University Medical School, Japan.

Find articles by Kuzuya, T. in: PubMed | Google Scholar

First Department of Medicine, Osaka University Medical School, Japan.

Find articles by Hori, M. in: PubMed | Google Scholar

First Department of Medicine, Osaka University Medical School, Japan.

Find articles by Taniguchi, N. in: PubMed | Google Scholar

First Department of Medicine, Osaka University Medical School, Japan.

Find articles by Kamada, T. in: PubMed | Google Scholar

First Department of Medicine, Osaka University Medical School, Japan.

Find articles by Tada, M. in: PubMed | Google Scholar

Published December 1, 1994 - More info

Published in Volume 94, Issue 6 on December 1, 1994
J Clin Invest. 1994;94(6):2193–2199. https://doi.org/10.1172/JCI117580.
© 1994 The American Society for Clinical Investigation
Published December 1, 1994 - Version history
View PDF
Abstract

Manganese superoxide dismutase (Mn-SOD) is induced in ischemic hearts 24 h after ischemic preconditioning, when tolerance to ischemia is acquired. We examined the relationship between Mn-SOD induction and the protective effect of preconditioning using cultured rat cardiac myocytes. Exposure of cardiac myocytes to brief hypoxia (1 h) decreased creatine kinase release induced by sustained hypoxia (3 h) that follows when the sustained hypoxia was applied 24 h after hypoxic preconditioning (57% of that in cells without preconditioning). The activity and content of Mn-SOD in cardiac myocytes were increased 24 h after hypoxic preconditioning (activity, 170%; content, 139% compared with cells without preconditioning) coincidentally with the acquisition of tolerance to hypoxia. Mn-SOD mRNA was also increased 20-40 min after preconditioning. Antisense oligodeoxyribonucleotides corresponding to the initiation site of Mn-SOD translation inhibited the increases in the Mn-SOD content and activity and abolished the expected decrease in creatine kinase release induced by sustained hypoxia after 24 h of hypoxic preconditioning. Sense oligodeoxyribonucleotides did not abolish either Mn-SOD induction or tolerance to hypoxia. These results suggest that the induction of Mn-SOD in myocytes by preconditioning plays a pivotal role in the acquisition of tolerance to ischemia at a later phase (24 h) of ischemic preconditioning.

Images.

Browse pages

Click on an image below to see the page. View PDF of the complete article

Version history
  • Version 1 (December 1, 1994): No description
Advertisement
Advertisement