Effects of interleukin-1 on syntheses of alkaline phosphatase, type X collagen, and 1,25-dihydroxyvitamin D3 receptor, and matrix calcification in rabbit chondrocyte cultures.

Y Kato; K Nakashima; M Iwamoto; H Murakami; H Hiranuma; T Koike; F Suzuki; H Fuchihata; Y Ikehara; M Noshiro

doi:10.1172/JCI116836

Department of Biochemistry, School of Dentistry, Hiroshima University, Japan.

Find articles by Kato, Y. in: PubMed | Google Scholar

Department of Biochemistry, School of Dentistry, Hiroshima University, Japan.

Find articles by Nakashima, K. in: PubMed | Google Scholar

Department of Biochemistry, School of Dentistry, Hiroshima University, Japan.

Find articles by Iwamoto, M. in: PubMed | Google Scholar

Department of Biochemistry, School of Dentistry, Hiroshima University, Japan.

Find articles by Murakami, H. in: PubMed | Google Scholar

Department of Biochemistry, School of Dentistry, Hiroshima University, Japan.

Find articles by Hiranuma, H. in: PubMed | Google Scholar

Department of Biochemistry, School of Dentistry, Hiroshima University, Japan.

Find articles by Koike, T. in: PubMed | Google Scholar

Department of Biochemistry, School of Dentistry, Hiroshima University, Japan.

Find articles by Suzuki, F. in: PubMed | Google Scholar

Department of Biochemistry, School of Dentistry, Hiroshima University, Japan.

Find articles by Fuchihata, H. in: PubMed | Google Scholar

Department of Biochemistry, School of Dentistry, Hiroshima University, Japan.

Find articles by Ikehara, Y. in: PubMed | Google Scholar

Department of Biochemistry, School of Dentistry, Hiroshima University, Japan.

Find articles by Noshiro, M. in: PubMed | Google Scholar

Published in Volume 92, Issue 5 on November 1, 1993
J Clin Invest. 1993;92(5):2323–2330. https://doi.org/10.1172/JCI116836.
© 1993 The American Society for Clinical Investigation

Published November 1, 1993 - Version history

The effect of IL-1 on expression of the mineralization-related phenotype by chondrocytes was examined. In cultures of rabbit growth plate chondrocytes, IL-1 beta at 0.1 ng/ml caused 95% decreases in alkaline phosphatase activity, alkaline phosphatase mRNA levels, the incorporation of 45Ca into insoluble material, and the calcium content during the hypertrophic stage. These effects of IL-1 beta were dose-dependent and were observed in 24-48 h. Furthermore, IL-1 beta suppressed increase in cell size and the syntheses of 1,25-dihydroxyvitamin D3 receptor and type X collagen, other markers of hypertrophy, but had little effect on the synthesis of total protein including type II collagen. The inhibition of calcification was observed only when chondrocytes were exposed to IL-1 before the onset of calcification: IL-1 treatment from the mineralization stage had a marginal effect on 45Ca incorporation into insoluble material. These results suggest that IL-1 inhibits chondrocyte hypertrophy and the onset of calcification in ossifying cartilage.

Images.

Version 1 (November 1, 1993): No description

Effects of interleukin-1 on syntheses of alkaline phosphatase, type X collagen, and 1,25-dihydroxyvitamin D3 receptor, and matrix calcification in rabbit chondrocyte cultures.

Y Kato, K Nakashima, M Iwamoto, H Murakami, H Hiranuma, T Koike, F Suzuki, H Fuchihata, Y Ikehara, and M Noshiro

Article tools

Metrics

Go to

Effects of interleukin-1 on syntheses of alkaline phosphatase, type X collagen, and 1,25-dihydroxyvitamin D3 receptor, and matrix calcification in rabbit chondrocyte cultures.

Y Kato, K Nakashima, M Iwamoto, H Murakami, H Hiranuma, T Koike, F Suzuki, H Fuchihata, Y Ikehara, and M Noshiro

Article tools

Metrics

Go to

Sign up for email alerts