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Free access | 10.1172/JCI110462

Elastase of U-937 Monocytelike Cells: COMPARISONS WITH ELASTASES DERIVED FROM HUMAN MONOCYTES AND NEUTROPHILS AND MURINE MACROPHAGELIKE CELLS

Robert M. Senior, Edward J. Campbell, Jill A. Landis, Fraya R. Cox, Charles Kuhn, and Hillel S. Koren

Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110

Department of Pathology, Washington University School of Medicine, St. Louis, Missouri 63110

Pulmonary Division, Department of Medicine, The Jewish Hospital of St. Louis, St. Louis, Missouri 63110

Division of Immunology, Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710

Find articles by Senior, R. in: JCI | PubMed | Google Scholar

Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110

Department of Pathology, Washington University School of Medicine, St. Louis, Missouri 63110

Pulmonary Division, Department of Medicine, The Jewish Hospital of St. Louis, St. Louis, Missouri 63110

Division of Immunology, Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710

Find articles by Campbell, E. in: JCI | PubMed | Google Scholar

Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110

Department of Pathology, Washington University School of Medicine, St. Louis, Missouri 63110

Pulmonary Division, Department of Medicine, The Jewish Hospital of St. Louis, St. Louis, Missouri 63110

Division of Immunology, Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710

Find articles by Landis, J. in: JCI | PubMed | Google Scholar

Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110

Department of Pathology, Washington University School of Medicine, St. Louis, Missouri 63110

Pulmonary Division, Department of Medicine, The Jewish Hospital of St. Louis, St. Louis, Missouri 63110

Division of Immunology, Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710

Find articles by Cox, F. in: JCI | PubMed | Google Scholar

Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110

Department of Pathology, Washington University School of Medicine, St. Louis, Missouri 63110

Pulmonary Division, Department of Medicine, The Jewish Hospital of St. Louis, St. Louis, Missouri 63110

Division of Immunology, Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710

Find articles by Kuhn, C. in: JCI | PubMed | Google Scholar

Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110

Department of Pathology, Washington University School of Medicine, St. Louis, Missouri 63110

Pulmonary Division, Department of Medicine, The Jewish Hospital of St. Louis, St. Louis, Missouri 63110

Division of Immunology, Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710

Find articles by Koren, H. in: JCI | PubMed | Google Scholar

Published February 1, 1982 - More info

Published in Volume 69, Issue 2 on February 1, 1982
J Clin Invest. 1982;69(2):384–393. https://doi.org/10.1172/JCI110462.
© 1982 The American Society for Clinical Investigation
Published February 1, 1982 - Version history
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Abstract

As an approach to facilitating the understanding of proteinases associated with monocytes we have studied U-937 monocytelike cells. Elastase activity was identified in U-937 cell extracts and compared to monocyte elastase activity, neutrophil elastase, and the elastase activity from a continuous line of murine macrophagelike cells (P388D1). Serine proteinase activity which solubilized 14C-labeled elastin accounted for >90% of the neutral proteinase activity of both U-937 cells and monocyte extracts. U-937 cell and monocyte elastase activities were similar catalytically, resembling neutrophil elastase. U-937 cells and monocytes showed other similarities: (a) both had activities reacting with [3H]diisopropylfluorophosphate that migrated in sodium dodecyl sulfate (SDS) polyacrylamide gels at ∼30,000 and 60,000 daltons and (b) both contained material that cross-reacted with antiserum raised to neutrophil elastase. Preliminary characterization of U-937 cell elastase activity by affinity chromatography and ion-exchange chromatography suggested the presence of at least two distinct elastases. Minimal elastase activity was found in U-937 cell-conditioned medium, indicating that the activity is not spontaneously released by the cells. In contrast to the elastase activity associated with U-937 cells and monocytes, the elastase activity associated with P388D1 cells was a metalloproteinase and was found principally in the culture medium. These results indicate (a) U-937 cells will be useful for further investigation of proteinases associated with normal monocytes; (b) monocytes and U-937 cells contain material with catalytic and immunologic similarities to neutrophil elastase; (c) monocyte elastase activity differs from elastase activity secreted by murine macrophages and murine macrophagelike cells of the P388D1 line.

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