Addition of increasing amounts of 125I-labeled desialylated thyroxine-binding globulin (DTBG) to hepatic cell membranes resulted in a progressive increase in binding. Saturability of membrane sites was indicated by a concentration beyond which further increases in [125I]DTBG resulted in no further binding. The binding curve for [125I]DTBG was similar to binding curves of desialylated orosomucoid, fetuin, and ceruloplasmin.
James S. Marshall, Allan M. Green, Jack Pensky, Stanford Williams, Arthur Zinn, Don M. Carlson
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