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Research Article Free access | 10.1172/JCI105558
Department of Medicine, Western Reserve University School of Medicine, Cleveland, Ohio
Metropolitan General Hospital, Cleveland, Ohio
†Research Career awardee of the U. S. Public Health Service (5-K6-HE-4576). Address requests for reprints to Dr. M. H. Kaplan, Dept. of Medicine, Metropolitan General Hospital, Cleveland, Ohio.
*Submitted for publication February 21, 1966; accepted December 22, 1966.
This work was presented in part at the meeting of the American Association of Immunologists, Atlantic City, N. J., April 11-16, 1966 (1).
This work was supported by research grant HE-03726 from the National Heart Institute, U. S. Public Health Service, and by a grant from the Kidney Foundation of Northeast Ohio.
Find articles by Krishnan, C. in: JCI | PubMed | Google Scholar
Department of Medicine, Western Reserve University School of Medicine, Cleveland, Ohio
Metropolitan General Hospital, Cleveland, Ohio
†Research Career awardee of the U. S. Public Health Service (5-K6-HE-4576). Address requests for reprints to Dr. M. H. Kaplan, Dept. of Medicine, Metropolitan General Hospital, Cleveland, Ohio.
*Submitted for publication February 21, 1966; accepted December 22, 1966.
This work was presented in part at the meeting of the American Association of Immunologists, Atlantic City, N. J., April 11-16, 1966 (1).
This work was supported by research grant HE-03726 from the National Heart Institute, U. S. Public Health Service, and by a grant from the Kidney Foundation of Northeast Ohio.
Find articles by Kaplan, M. in: JCI | PubMed | Google Scholar
Published April 1, 1967 - More info
The γG-globulin eluted at acid pH from kidney cortex homogenates and isolated glomeruli of five of six patients with lupus nephritis was found to exhibit antinuclear activity, which was not dependent on presence of fresh human serum. Specificity, as demonstrated by absorption of antinuclear activity, was related to nucleoprotein in three glomerular acid eluates and to DNA in two acid eluates as well as in a deoxyribonuclease digest of disrupted glomeruli in one patient. Antinuclear activity was not found in acid eluates of kidneys from two patients with chronic liver disease and chronic discoid lupus, respectively, and one with lupus nephritis. These patients had a low titer of serum antinuclear factor and lesser amounts of kidney bound immunoglobulins. The presence of antinuclear activity in eluates of kidneys appeared to correlate with the amount of glomerular bound immunoglobulin and the level of antinuclear antibodies in serum. These findings suggest that in lupus nephritis, part of the glomerular bound immunoglobulin is derived from serum antinuclear factors possibly deposited as immune complexes.
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