[HTML][HTML] Targeted construction of phosphorylation-independent β-arrestin mutants with constitutive activity in cells
A Kovoor, J Celver, RI Abdryashitov, C Chavkin… - Journal of Biological …, 1999 - ASBMB
Arrestin proteins play a key role in the desensitization of G protein-coupled receptors
(GPCRs). Recently we proposed a molecular mechanism whereby arrestin preferentially
binds to the activated and phosphorylated form of its cognate GPCR. To test the model, we
introduced two different types of mutations into β-arrestin that were expected to disrupt two
crucial elements that make β-arrestin binding to receptors phosphorylation-dependent. We
found that two β-arrestin mutants (Arg 169→ Glu and Asp 383→ Ter)(Ter, stop codon) are …
(GPCRs). Recently we proposed a molecular mechanism whereby arrestin preferentially
binds to the activated and phosphorylated form of its cognate GPCR. To test the model, we
introduced two different types of mutations into β-arrestin that were expected to disrupt two
crucial elements that make β-arrestin binding to receptors phosphorylation-dependent. We
found that two β-arrestin mutants (Arg 169→ Glu and Asp 383→ Ter)(Ter, stop codon) are …