Protein kinase C isotypes in human erythroleukemia (K562) cell proliferation and differentiation. Evidence that beta II protein kinase C is required for proliferation
NR Murray, GP Baumgardner, DJ Burns… - Journal of Biological …, 1993 - ASBMB
The human erythroleukemia (K562) cell line undergoes megakaryocytic differentiation and
cessation of proliferation when treated with phorbol myristate acetate (PMA). To investigate
the role of individual protein kinase C (PKC) isotypes in these events, we have assessed
PKC isotype expression during leukemic proliferation and PMA-induced differentiation.
Immunoblot analysis using isotype-specific antibodies demonstrates that proliferating K562
cells express the alpha, beta II, and zeta PKC isotypes. PMA-induced differentiation and …
cessation of proliferation when treated with phorbol myristate acetate (PMA). To investigate
the role of individual protein kinase C (PKC) isotypes in these events, we have assessed
PKC isotype expression during leukemic proliferation and PMA-induced differentiation.
Immunoblot analysis using isotype-specific antibodies demonstrates that proliferating K562
cells express the alpha, beta II, and zeta PKC isotypes. PMA-induced differentiation and …