[HTML][HTML] Inhibition of translocation of nascent apolipoprotein B across the endoplasmic reticulum membrane is associated with selective inhibition of the synthesis of …
M Pan, J Liang, EA Fisher, HN Ginsberg - Journal of Biological Chemistry, 2000 - ASBMB
In HepG2 cells, inhibition of apolipoprotein B100 (apoB) translocation across the
endoplasmic reticulum by an microsomal triglyceride transfer protein (MTP) inhibitor (CP-
10447) in the presence ofN-acetyl-leucinyl-norleucinal, a proteasomal inhibitor, results in
accumulation of newly synthesized apoB in the translocation channel. Here we
demonstrated that such accumulation led to a specific reduction of apoB synthesis. ApoB
mRNA levels remained unchanged, but we observed reduced rates of elongation of nascent …
endoplasmic reticulum by an microsomal triglyceride transfer protein (MTP) inhibitor (CP-
10447) in the presence ofN-acetyl-leucinyl-norleucinal, a proteasomal inhibitor, results in
accumulation of newly synthesized apoB in the translocation channel. Here we
demonstrated that such accumulation led to a specific reduction of apoB synthesis. ApoB
mRNA levels remained unchanged, but we observed reduced rates of elongation of nascent …