Tetramer-guided epitope mapping: a rapid approach to identify HLA-restricted T-cell epitopes from composite allergens
LLD Archila, WW Kwok - Food Allergens: Methods and Protocols, 2017 - Springer
LLD Archila, WW Kwok
Food Allergens: Methods and Protocols, 2017•SpringerTetramer-guided epitope mapping (TGEM) is a technique in immunology that permits the
rapid identification of allergenic epitopes through peptide screening procedures utilizing
human lymphocyte antigen (HLA) class II tetramers as staining reagents for detection. The
identification of allergenic epitopes is a prerequisite for the accurate characterization of
allergen-specific CD4+ T cells without in vitro stimulation. Additionally, these MHC-II/peptide
complexes that interact with T-cell receptors (TCR) of pathogenic CD4+ T cells are …
rapid identification of allergenic epitopes through peptide screening procedures utilizing
human lymphocyte antigen (HLA) class II tetramers as staining reagents for detection. The
identification of allergenic epitopes is a prerequisite for the accurate characterization of
allergen-specific CD4+ T cells without in vitro stimulation. Additionally, these MHC-II/peptide
complexes that interact with T-cell receptors (TCR) of pathogenic CD4+ T cells are …
Abstract
Tetramer-guided epitope mapping (TGEM) is a technique in immunology that permits the rapid identification of allergenic epitopes through peptide screening procedures utilizing human lymphocyte antigen (HLA) class II tetramers as staining reagents for detection. The identification of allergenic epitopes is a prerequisite for the accurate characterization of allergen-specific CD4+ T cells without in vitro stimulation. Additionally, these MHC-II/peptide complexes that interact with T-cell receptors (TCR) of pathogenic CD4+ T cells are compatible with a different number of assays like Intracelullar Cytokine Staining (ICS), and Carboxyfluorescein succinimidyl ester (CFSE) making it a robust technology to study the functionality of allergen-specific CD4+ T cells.
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