Whole-mount in situ hybridizations on zebrafish embryos using a mixture of digoxigenin- and fluorescein-labelled probes.
T Jowett, L Lettice - 1994 - cabidigitallibrary.org
T Jowett, L Lettice
1994•cabidigitallibrary.orgThis item in the" Technical Tips" section of the journal describes protocols for differentially
recognizing any 2 probes in zebrafish embryos. Both methods require that antisense RNA
probes are differentially labelled with digoxigenin-UDP and fluorescein-UTP. The probes
are hybridized simultaneously but visualized sequentially.
recognizing any 2 probes in zebrafish embryos. Both methods require that antisense RNA
probes are differentially labelled with digoxigenin-UDP and fluorescein-UTP. The probes
are hybridized simultaneously but visualized sequentially.
Abstract
This item in the "Technical Tips" section of the journal describes protocols for differentially recognizing any 2 probes in zebrafish embryos. Both methods require that antisense RNA probes are differentially labelled with digoxigenin-UDP and fluorescein-UTP. The probes are hybridized simultaneously but visualized sequentially.
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