Protein engineering of protein kinase A catalytic subunits results in the acquisition of novel inhibitor sensitivity
CM Niswender, RW Ishihara, LM Judge… - Journal of Biological …, 2002 - ASBMB
Analysis of the role of specific protein kinases in signal transduction networks has relied
heavily on ATP analog inhibitors. Currently used agents, however, often do not distinguish
between kinase family members. Genetic approaches can also be used to inactivate a
specific kinase, but these techniques do not afford the rapid kinetics possible with
pharmacological inhibitors. To circumvent this problem, modification of the structure of a
particular protein kinase can be performed to engineer a drug-target interaction of choice …
heavily on ATP analog inhibitors. Currently used agents, however, often do not distinguish
between kinase family members. Genetic approaches can also be used to inactivate a
specific kinase, but these techniques do not afford the rapid kinetics possible with
pharmacological inhibitors. To circumvent this problem, modification of the structure of a
particular protein kinase can be performed to engineer a drug-target interaction of choice …
