Accurate determination of local defocus and specimen tilt in electron microscopy
JA Mindell, N Grigorieff - Journal of structural biology, 2003 - Elsevier
Accurate knowledge of defocus and tilt parameters is essential for the determination of three-
dimensional protein structures at high resolution using electron microscopy. We present two
computer programs, CTFFIND3 and CTFTILT, which determine defocus parameters from
images of untilted specimens, as well as defocus and tilt parameters from images of tilted
specimens, respectively. Both programs use a simple algorithm that fits the amplitude
modulations visible in a power spectrum with a calculated contrast transfer function (CTF) …
dimensional protein structures at high resolution using electron microscopy. We present two
computer programs, CTFFIND3 and CTFTILT, which determine defocus parameters from
images of untilted specimens, as well as defocus and tilt parameters from images of tilted
specimens, respectively. Both programs use a simple algorithm that fits the amplitude
modulations visible in a power spectrum with a calculated contrast transfer function (CTF) …